Important aspects of Demodex diagnostics

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Alexey Kubanov A, Gallyamova Yulia, Anzhela Grevtseva


The article presented our reviews on methodological advances in the diagnosis of demodicosis via our own research on demodicosis diagnosis efficiency with the aid of fluorescence lifetime measurement using confocal laser scanning microscopy. Under our supervision, there were 60 patients with acne and rosacea complicated with demodicosis, 60 patients with acne and rosacea without demodicosis, and 30 healthy volunteers. All patients underwent skin scraping and epilation of eyebrows and/or eyelashes, and the examination of skin morphology was conducted using confocal laser scanning microscopy. The research has shown the advantages of in vivo confocal laser scanning microscopy over the conventional microscope.


Demodex; demodicosis; Demodex mites; confocal laser scanning microscopy; acne; rosacea

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Chen W, Plewig G. Human demodecosis: Revisit and a pro¬posed classification. Br J Dermatol 2014; 170(6): 1219– 1225. doi: 10.1111/bjd.12850.

Whiting DA. Diagnostics and predictive value of horizon-tal sections of scalp biopsy specimen in male pattern an-drogenic alopecia. J Am Acad Dermatol 1993; 28(5 Part 1): 755–763. doi: 10.1016/0190-9622(93)70106-4.

Akilov OE, Butov YS, Mamcuoglu KY. A clinic-patholo-gical approach to the classification of human demodecosis. J Dtsch Dermatol Ges 2005; 3(8): 607–614. doi: 10.1111/ j.1610-0387.2005.05725.x.

Sirmays NS, Abesadze GA, Ustinov MV. Demodekoz: patogeneticheskie aspekty pri razlichnykh dermatozakh litsa: Metodicheskoe posobie (Russian) [Demodecosis: pathogenic aspects in various facial dermatoses: Methodo-logical guidelines]. Moscow: Gel’tek-Medika; 2013. p. 26.

Crawford GH, Pelle MT, James WD. Rosacea: I. Etiology, pathogenesis, and subtype classification. J Am Acad Der-matol 2004; 51(3): 327–341. doi: 10.1016/j.jaad.2004.03. 030.

Bonnar E, Eustace P, Powell FC. Demodex mite in nor-mal skin. Lancet 1991; 337(8750): 1168. doi: 10.1016/ 0140-6736(91)92841-O.

Segal R, Mimouni D, Feuerman H, Pagovitz O, Da¬vid M. Report: Dermoscopy as a diagnostic tool in demodicidosis. Int J Dermatol 2010; 49(9): 1018–1023. doi: 10.1111/ j.1365-4632.2010.04495.x.

Astner S, Dietterle S, Otberg N, Röwert-Huber HJ, Sto¬¬ck¬-fleth E, et al. Clinical applicability of in vivo fluorescence confocal microscopy for noninvasive diagnosis and thera-peutic monitoring of nonmelanoma skin cancer. J Biomed Opt 2008; 13(1): 014003. doi: 10.1117/1.2837411.

Psaty EL, Halpern AC. Current and emerging technologies in melanoma diagnosis: The state of the art. Clin Dermatol 2009; 27(1): 35–45. doi: 10.1016/j.clindermatol.2008.09. 004.

Rajadhyaksha M. Confocal reflectance microscopy: Diag-nosis of skin cancer without biopsy? In: National Academy of Engineering (editors). Frontiers of Engineering: Reports on Leading Edge Engineering From the 1998 NAE Sym-posium on Frontiers of Engineering. Washington: National Academies Press; 1999. p. 24–33. doi: 10.17226/6411.

Nwaneshiudu A, Kuschal C, Sakamoto FH, Anderson RR, Schwarzenberger K, et al. Introduction to confocal mi-croscopy. J Invest Dermatol 2012; 132(12): 1–5. doi: 10. 1038/jid.2012.429.

Kubanova AA, Chikin VV, Shtirshneider YY, Katunina OR. Confocal laser scanning microscopy in vivo for diagnosing melanocytic skin neoplasms. Vestnik dermatologii i venerologii 2014; 3: 85–94.

Neerken S, Lucassen GW, Lenderink E, Nuijs TAM. In vivo imaging of human skin: A comparison of optical co-herence tomography and confocal laser scanning micros-copy. In: Tuchin VV, Izatt JA, Fujimoto JG (editors). Proc. SPIE 4956, Coherence Domain Optical Methods and Op-tical Coherence Tomography in Biomedicine VII, 299; 2003 Jan 25; San Jose CA. 2003. doi: 10.1117/12.478948.

Messmer EM, Torres Suárez E, Mackert MI, Zapp DM, Kampik A. In vivo confocal microscopy in blepharitis. Klin Monbl Augenheilkd 2005; 222(11): 894–900. doi: 10.1055/ s-2005-858798.

Nori S, Rius-Diaz F, Cuevas J, Goldgeier M, Jaen P, et al. Sensitivity and specificity of reflectance-mode confocal microscopy for in vivo diagnosis of basal cell carcinoma: a multicenter study. J Am Acad Dermatol 2004; 51(6): 923– 930. doi: 10.1016/j.jaad.2004.06.028.

Gerger A, Koller S, Kern T, Massone C, Steigner K, et al. Diagnostic applicability of in vivo confocal laser scanning microscopy in melanocytic skin tumors. J Invest Dermatol 2005; 124(3): 493–498. doi: 10.1111/j.0022-202X.2004. 23569.x.

Gerger A, Koller S, Weger W, Richtig E, Kerl H, et al. Sensitivity and specificity of confocal laser-scanning mi-croscopy for in vivo diagnosis of malignant skin tumors. Cancer 2006; 107(1): 193–200. doi: 10.1002/cncr.21910.

Shtirshnayder YY, Michenko AV, Katunina OR, Zubarev AR. New non-invasive imaging technology in dermatology. Vestnik dermatologii i venerologii 2011; 5: 41–53.

Sattler EC, Maier T, Hoffmann VS, Hegyi J, Ruzicka T, et al. Noninvasive in vivo detection and quantification of Demodex mites by confocal laser scanning microscopy. Br J Dermatol 2012; 167(5): 1042–1047. doi: 10.1111/ j.1365-2133.2012.11096.x.

Kojima T, Ishida R, Sato EA, Kawakita T, Ibrahim OMA, et al. In vivo evaluation of ocular demodecosis using laser scanning confocal microscopy. Invest Ophthalmol Vis Sci 2011; 52(1): 565–569. doi: 10.1167/iovs.10-5477.

Erdemir AT, Gurel MS, Koku Aksu AE, Bilgin Karahalli F, Incel P, et al. Reflectance confocal microscopy vs. stand-ardized skin surface biopsy for measuring the density of Demodex mites. Skin Res Technol 2014; 20(4): 435–439. doi: 10.1111/srt.12137.


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